Cell-free protein synthesis: applications come of age ED Carlson, R Gan, CE Hodgman, MC Jewett Biotechnology advances 30 (5), 1185-1194, 2012 | 636 | 2012 |
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement FJ Isaacs, PA Carr, HH Wang, MJ Lajoie, B Sterling, L Kraal, AC Tolonen, ... Science 333 (6040), 348-353, 2011 | 590 | 2011 |
How many human proteoforms are there? R Aebersold, JN Agar, IJ Amster, MS Baker, CR Bertozzi, ES Boja, ... Nature chemical biology 14 (3), 206-214, 2018 | 424 | 2018 |
Cell-free synthetic biology: thinking outside the cell CE Hodgman, MC Jewett Metabolic engineering 14 (3), 261-269, 2012 | 411 | 2012 |
Mimicking the Escherichia coli cytoplasmic environment activates long‐lived and efficient cell‐free protein synthesis MC Jewett, JR Swartz Biotechnology and bioengineering 86 (1), 19-26, 2004 | 403 | 2004 |
An integrated cell‐free metabolic platform for protein production and synthetic biology MC Jewett, KA Calhoun, A Voloshin, JJ Wuu, JR Swartz Molecular systems biology 4 (1), 220, 2008 | 354 | 2008 |
High-throughput preparation methods of crude extract for robust cell-free protein synthesis YC Kwon, MC Jewett Scientific reports 5 (1), 1-8, 2015 | 275 | 2015 |
Cell‐free metabolic engineering: biomanufacturing beyond the cell QM Dudley, AS Karim, MC Jewett Biotechnology journal 10 (1), 69-82, 2015 | 266 | 2015 |
Cell-free gene expression: an expanded repertoire of applications AD Silverman, AS Karim, MC Jewett Nature Reviews Genetics 21 (3), 151-170, 2020 | 236 | 2020 |
Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids M Amiram, AD Haimovich, C Fan, YS Wang, HR Aerni, I Ntai, DW Moonan, ... Nature biotechnology 33 (12), 1272-1279, 2015 | 211 | 2015 |
Impact of systems biology on metabolic engineering of Saccharomyces cerevisiae J Nielsen, MC Jewett FEMS yeast research 8 (1), 122-131, 2008 | 205 | 2008 |
The genome-scale metabolic model iIN800 of Saccharomyces cerevisiae and its validation: a scaffold to query lipid metabolism I Nookaew, MC Jewett, A Meechai, C Thammarongtham, K Laoteng, ... BMC systems biology 2 (1), 1-15, 2008 | 197 | 2008 |
Protein synthesis by ribosomes with tethered subunits C Orelle, ED Carlson, T Szal, T Florin, MC Jewett, AS Mankin Nature 524 (7563), 119-124, 2015 | 183 | 2015 |
Linking high-resolution metabolic flux phenotypes and transcriptional regulation in yeast modulated by the global regulator Gcn4p JF Moxley, MC Jewett, MR Antoniewicz, SG Villas-Boas, H Alper, ... Proceedings of the National Academy of Sciences 106 (16), 6477-6482, 2009 | 180 | 2009 |
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery AS Karim, MC Jewett Metabolic engineering 36, 116-126, 2016 | 171 | 2016 |
Update on designing and building minimal cells MC Jewett, AC Forster Current opinion in biotechnology 21 (5), 697-703, 2010 | 147 | 2010 |
In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation MC Jewett, BR Fritz, LE Timmerman, GM Church Molecular systems biology 9 (1), 678, 2013 | 141 | 2013 |
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids RW Martin, BJ Des Soye, YC Kwon, J Kay, RG Davis, PM Thomas, ... Nature communications 9 (1), 1-9, 2018 | 137 | 2018 |
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery T Jaroentomeechai, JC Stark, A Natarajan, CJ Glasscock, LE Yates, ... Nature communications 9 (1), 1-11, 2018 | 136 | 2018 |
Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation SH Hong, I Ntai, AD Haimovich, NL Kelleher, FJ Isaacs, MC Jewett ACS synthetic biology 3 (6), 398-409, 2014 | 134 | 2014 |